Ndị na-adọta ike dị eluDMPTnaDMTbụ ihe ọhụrụ na-adọrọ adọrọ maka anụ mmiri. N'ime ọmụmụ ihe a, ndị na-adọrọ mmasị dị eluDMPTnaDMTagbakwunyere na nri carp iji nyochaa mmetụta ndị na-adọrọ mmasị abụọ ahụ na-eri nri na nkwalite uto. Nsonaazụ gosiri na mgbakwunye nke ndị na-adọrọ mmasị dị eluDMPTnaDMTna nri na-abawanye ụbara ugboro ole azụ nke nnwale ahụ ma nwee mmetụta nri dị ukwuu; N'otu oge ahụ, mgbakwunye nke ntinye dị iche iche nke ndị na-adọrọ mmasị dị eluDMPTnaDMTna nri nri na-abawanye ụbara ibu arọ, ọnụego uto akọwapụtara, na ọnụego nlanarị nke azụ nnwale, ebe ọnụọgụ nri belatara nke ukwuu. Nsonaazụ nyocha gosikwara nke ahụDMPTnwere mmetụta dị ịrịba ama na-adọta na ịkwalite uto nke carp ma e jiri ya tụnyereDMT.

Ihe na-adọta nri anụ mmiri bụ ihe mgbakwunye na-adịghị edozi ahụ. Ịgbakwunye ndị na-adọrọ mmasị na-azụ azụ nwere ike ịkwalite nri ha nke ọma, mụbaa nri ha na-eri, belata nri fọdụrụnụ na mmiri, wee si otú ahụ belata mmetọ nke mmiri aquaculture.DMPTnaDMTbụ ihe na-arụsi ọrụ ike n'ọtụtụ ebe n'ime ihe ndị dị n'oké osimiri, na-eje ozi dị ka ndị na-enye onyinye methyl dị irè na ndị na-achịkwa nrụgide osmotic dị mkpa. Ha nwekwara mmetụta dị ukwuu na-enye nri na uto na-akwalite anụmanụ ndị dị n'ime mmiri.

Mgbe ha mechara ọmụmụ ihe dị mkpa gbasara anụmanụ ndị dị n'ime mmiri dị ka crucian carp, red snapper, goldfish, na shrimp hụrụ n'anya, ndị nchọpụta Japan chọpụtara naDMPTnaDMTnwere mmetụta dị mma na-adọrọ adọrọ na azụ mmiri na mmiri, crustaceans, na shellfish. Na-agbakwunye ntanye dị ala nke ndị na-adọrọ mmasị dị eluDMPTnaDMTna nri nwere ike mee ngwangwa nri na uto nke mmiri dị iche iche na azụ mmiri dị n'oké osimiri. N'ime nnwale a, ndị na-adọta ike dị eluDMPTnaDMTagbakwunyere na nri azu kaapu iji mụọ mmetụta ha na nri azu kaapu na nkwalite uto, na-enye data ntụaka maka ngwa zuru ebe niile nke ihe ọhụrụ abụọ a na-adọrọ adọrọ na ụlọ ọrụ nri na aquaculture.

1 Akụrụngwa na Ụzọ

1.1 Ihe nnwale na azụ nnwale
S. S' - Dimethylacetic acid thiazole (DMT), DMPT
Ewepụtara kap ahụ nnwale ahụ n'ugbo aquaculture, nwere ahụ dị mma yana nkọwapụta nke ọma. Tupu nnwale ahụ amalite n'ihu ọha, a ga-azụta azụ̀ nnwale ahụ nwa oge n'ụlọ nyocha maka ụbọchị 7, n'oge nke a ga-enye nri kapịrị ọnụ nke ụlọ ọrụ nri na-enye.
1.2 Nri nnwale
1.2.1 Nri ule nlegharị anya: Ghichaa ihe oriri carp nke ụlọ ọrụ mmepụta ihe na-enye, tinye nha nha A-starch, gwakọta ya nke ọma, ma jikọta ya na mmiri mmiri dị nro kwesịrị ekwesị iji mee 5g bọọlụ nnyapade nke ọ bụla dị ka nri otu njikwa. N'otu oge ahụ, kwadebe nri nri nri site na mbụ ịzọpịa nri azu kaapu, na-agbakwunye alfa starch hà nhata, na ịgbakwunye bait DMT naDMPTna mkpokọta abụọ nke 0.5g / kg na 1g / kg, n'otu n'otu. Gwakọta nke ọma ma gwakọta ya na mmiri a na-ekpo ọkụ kwesịrị ekwesị iji mee ka bọlbụ 5g ọ bụla na-adọrọ adọrọ.
1.2.2 ndepụta ule uto:

Ghichaa nri carp (site n'otu ebe ahụ dị n'elu) n'ime ntụ ntụ, gafere ya na sieve 60 mesh, tinye nha nha nke starch alpha, gwakọta nke ọma, gwakọta ya na mmiri a na-ekpo ọkụ, gbanye ya na sieve n'ime granules, na ikuku kpoo ya iji nweta nri ndị na-achịkwa maka ule uto. The synthesizedDMTna kristal DMPT gbazere n'ime mmiri distilled iji kwadebe ihe ngwọta nke ntinye uche kwesịrị ekwesị, nke a na-eji gwakọta nri carp na starch agwakọta nke ọma na granules. Mgbe ihicha, enwetara nri otu nnwale ahụ, yaDMTna DMPT agbakwunyere na gradients atọ nke 0.1g/kg, 0.2g/kg, na 0.3g/kg, n'otu n'otu.

1.3 Usoro ule
1.3.1 Nnwale nlegharị anya: Họrọ carp nnwale 5 (nwere nkezi ịdị arọ nke 30g) dị ka azụ ule. Tupu ule ahụ, gụọ agụụ maka awa 24, wee tinye azụ a na-anwale na aquarium iko (nke nwere nha 40 × 30 × 25cm). A na-edozi nri a na-erite n'ebe dị anya nke 5.0cm site na ala nke aquarium na-eji ahịrị a kwụsịtụrụ na mmanya kwụsiri ike. Azụ ahụ na-ata ụgbụ ahụ ma na-ama jijiji n'ahịrị, nke a na-ebunye ya na ogwe kwụ ọtọ ma na-edekọ ya site na onye na-edekọ wiil. A na-agbakọ ugboro ole ịta ụgbụ na-adabere na mmaji kacha elu nke 5 nwale azụ na-ata azụ n'ime nkeji abụọ. A na-emegharị ule nri maka otu nri ọ bụla ugboro atọ, na-eji bọọlụ nrapado nri a kwadebere ọhụrụ oge ọ bụla. Site na-eduzi ule ugboro ugboro iji nweta ngụkọta ọnụ ọgụgụ na nkezi ugboro nke baiting, nri mmetụta nkeDMTna DMPT na azu kaapu nwere ike inyocha.

1.3.2 Nnwale uto na-eji aquariums iko 8 (nha 55 × 45 × 50cm), nke nwere omimi mmiri nke 40cm, okpomọkụ mmiri eke, na mmụba na-aga n'ihu. A na-ekenye azụ ndị a na-anwale na-enweghị usoro wee kewaa ụzọ abụọ maka nnwale ahụ. Otu nke mbụ nwere aquarium anọ, ọnụ ọgụgụ X1 (otu njikwa), X2 (0.1gDMT / kg nri), X3 (0.2gDMT / kg nri), X4 (0.3gDMT / kg nri); Otu ọzọ nke 4 aquariums, ọnụ Y1 (otu njikwa), Y2 (0.10g DMPT / kg nri), Y3 (0.2g DMPT / kg nri), Y4 (0.30g DMPT / kg nri). Azụ 20 n'otu igbe, a na-enye nri ugboro atọ n'ụbọchị na 8:00, 13:00, na 17:00, yana ọnụego nri kwa ụbọchị nke 5-7% nke ibu ahụ. Nnwale ahụ were izu isii. Na mmalite na njedebe nke nnwale ahụ, a tụrụ oke mmiri mmiri nke azụ ule wee dekọọ ọnụego nlanarị nke otu ọ bụla.

2.1 Mmetụta nri nke DMPT naDMTna carp
Mmetụta nri nke DMPT naDMTna azu azu a na-egosiputa site na ntakiri ntakiri nke azu nnwale n'oge nnwale nke nkeji 2, dika egosiri na Tebụl 1. Nnwale ahụ chọpụtara na mgbe agbakwunyere DMPT na nri DMT na aquarium, azụ nnwale gosipụtara ngwa ngwa omume nri nri na-arụsi ọrụ ike, ebe mgbe ị na-eji nri nri otu njikwa, mmeghachi omume nke azụ nnwale ahụ dị ntakịrị. Tụnyere ndepụta njikwa, azụ nnwale ahụ nwere mmụba dị ukwuu na ugboro ole a na-ata nri nnwale ahụ. DMT na DMPT nwere mmetụta na-adọrọ adọrọ na kapịtị nnwale.

Ọnụọgụ uru dị arọ, ọnụ ọgụgụ uto akọwapụtara, na ọnụ ọgụgụ nlanarị nke carp nyere nri dị iche iche nke DMPT ka abawanyela nke ukwuu ma e jiri ya tụnyere ndị ejiri nri njikwa nri, ebe ọnụọgụ nri belatara nke ukwuu. N'ime ha, mgbakwunye DMPT na T2, T3, na T4 mụbara uru ịdị arọ kwa ụbọchị nke otu atọ site na 52.94%, 78.43%, na 113.73%, n'otu n'otu, ma e jiri ya tụnyere otu njikwa. Ọnụego ịdị arọ nke T2, T3, na T4 mụbara site na 60.44%, 73.85%, na 98.49%, n'otu n'otu, ọnụego uto a kapịrị ọnụ mụbara site na 41.22%, 51.15%, na 60.31%, n'otu n'otu. Ọnụego nlanarị ahụ gbagoro site na 90% ruo 95%, yana ọnụ ọgụgụ nri belatara site na 28.01%, 29.41%, na 33.05%, n'otu n'otu.

3. Nkwubi okwu

Na nnwale a, maDMTma ọ bụ agbakwunyere DMPT, ugboro nri, ọnụego uto akọwapụtara, na uru ịdị arọ kwa ụbọchị nke azụ nnwale n'ime otu ọ bụla abawanyela nke ukwuu ma e jiri ya tụnyere ndị na-ahụ maka njikwa, ebe ọnụọgụ nri belatara nke ukwuu. Ma ọ bụ DMT ma ọ bụ DMPT, mmetụta na-akwalite uto na-abawanye ụba na mmụba nke ego mgbakwunye na ntinye atọ nke 0.1g/kg, 0.2g/kg, na 0.3g/kg. N'otu oge ahụ, e mere ntụnyere nri na nkwalite uto nke DMT na DMPT. Achọpụtara na n'okpuru otu ntinye ntutu isi, ugboro nri, oke ibu, na ọnụ ọgụgụ dị iche iche na-eto eto nke azụ nwale na nri nri DMPT na-abawanye nke ukwuu ma e jiri ya tụnyere ìgwè nri DMT, ebe ọnụọgụ nri na-ebelata nke ukwuu. N'ikwu okwu n'otu n'otu, DMPT nwere mmetụta dị ịrịba ama na ịdọta na ịkwalite uto nke carp ma e jiri ya tụnyere DMT. Nnwale a jiri DMPT na DMT agbakwunyere na nri carp iji nyochaa nri na mmetụta na-akwalite uto ha. Nsonaazụ gosiri na DMPT na DMT nwere atụmanya ngwa sara mbara dị ka ọgbọ ọhụrụ nke ihe na-adọta anụ mmiri.