Ihe ndị na-adọta ike dị eluDMPTnaDMTbụ ihe ọhụrụ na ihe ndị na-adọta anụmanụ mmiri nke ọma. N'ọmụmụ ihe a, ihe ndị na-adọta ike dị eluDMPTnaDMTA tinyere ya na nri azụ̀ carp iji chọpụta mmetụta nke ihe abụọ ahụ na-adọta n'ahụ nri azụ̀ carp na ịkwalite uto. Nsonaazụ gosiri na mgbakwunye nke ihe ndị na-adọta n'ahụ ike dị elu.DMPTnaDMTna nri ahụ mụbara ugboro ole azụ̀ nnwale ahụ na-ata ata nke ukwuu ma nwee mmetụta dị ukwuu n'inye nri; N'otu oge ahụ, mgbakwunye nke ọtụtụ ihe ndị na-adọta ike dị elu dị iche icheDMPTnaDMTMaka nri ahụ, ọ mụbara ọnụego ibu, ọnụego uto kpọmkwem, na ọnụego ndụ nke azụ nnwale ahụ nke ukwuu, ebe ọnụọgụ nri ahụ belatara nke ukwuu. Nsonaazụ nnyocha ahụ gosikwara naDMPTnwere mmetụta dị ukwuu karị n'ịdọta ma kwalite uto nke carp ma e jiri ya tụnyereDMT.

Ihe na-adọta nri anụmanụ n'ime mmiri abụghị ihe mgbakwunye na-edozi ahụ. Ịtinye ihe ndị na-adọta azụ n'ime azụ nwere ike ịkwalite nri ha nke ọma, mee ka nri ha dịkwuo mma, belata ihe fọdụrụ n'ime mmiri, ma si otú a belata mmetọ na mmiri aquaculture.DMPTnaDMTbụ ihe ndị na-arụ ọrụ nke ọma dị n'ime ihe ndị dị ndụ n'ime mmiri, na-eje ozi dị ka ndị na-enye methyl dị irè na ndị na-achịkwa nrụgide osmotic dị mkpa. Ha nwekwara mmetụta dị ukwuu n'inye nri na mmụba uto n'ahụ anụmanụ mmiri.

Mgbe ha mechara nnyocha ndị dị mkpa gbasara anụmanụ mmiri dị ka crucian carp, red snapper, goldfish, na spotted oporo, ndị nchọpụta Japan chọpụtara naDMPTnaDMTnwere mmetụta dị mma n'ahụ azụ̀ mmiri dị ọhụrụ na nke mmiri, crustaceans, na azụ̀ shellfish. Na-eme ka obere ihe ndị na-adọta ike dị elu dịkwuo elu.DMPTnaDMTn'ime nri nwere ike ime ka nri na uto nke azụ dị iche iche dị ọhụrụ na nke mmiri dịkwuo ngwa ngwa. Na nnwale a, ihe ndị na-adọta ike dị eluDMPTnaDMTE tinyere nri azụ̀ kapị iji mụọ mmetụta ha nwere n'inye azụ̀ kapị na ịkwalite uto, na-enye data ntụaka maka ojiji zuru oke nke ihe abụọ a na-adọta ndị mmadụ ọhụrụ n'ụlọ ọrụ nri na ịkụ azụ̀.

1 Ihe na Usoro

1.1 Ihe nnwale na azụ nnwale
S. S' - Dimethylacetic acid thiazole (DMT), DMPT
E si n'ugbo aku na-azụ azụ̀ wepụta azụ̀ a na-eme nnwale, nke nwere ahụ dị mma na nkọwapụta dị mma. Tupu nnwale ahụ amalite n'ihu ọha, a ga-azụ azụ̀ a nwa oge n'ime ụlọ nyocha ruo ụbọchị asaa, ebe a ga-enye ha nri azụ̀ a na-azụ site n'ụlọ ọrụ mmepụta nri.
1.2 Njem nnwale
1.2.1 Nri nnwale ihe ndọta: Ghichaa nri carp nke ụlọ ọrụ nri na-enye, tinye otu ego nke A-starch, gwakọta ya nke ọma, ma gwakọta ya na mmiri kwesịrị ekwesị iji mee bọọlụ 5g nke ọ bụla ka otu njikwa na-eri nri. N'otu oge ahụ, kwadebe nri azụ site na ịgwepịa nri carp, tinye otu ego nke alfa starch, na itinye nri DMT naDMPTna mkpokọta abụọ nke 0.5g/kg na 1g/kg, n'otu n'otu. Gwakọta ya nke ọma ma gwakọta ya na mmiri kwesịrị ekwesị iji mee ka bọọlụ 5g ọ bụla na-arapara arapara.
1.2.2 Nnwale uto:

Ghichaa nri carp (site na otu isi iyi ahụ dị n'elu) ka ọ bụrụ ntụ, gafere ya site na sieve mesh 60, tinye otu ego nke alpha starch, gwakọta nke ọma, gwakọta ya na mmiri a gwakọtara agwakọta, pịa ya site na sieve ka ọ bụrụ granules, ma kpoo ya ikuku iji nweta nri otu njikwa maka ule uto.DMTa gbazere kristal DMPT n'ime mmiri a gwakọtara agwakọta iji kwadebe ihe ngwọta nke oke njupụta kwesịrị ekwesị, nke e ji gwakọta nri carp na starch agwakọtara nke ọma ka ọ bụrụ granules. Mgbe ọ kpọchara, e nwetara nri otu nnwale ahụ, ya naDMTna DMPT tinyere na gradients atọ nke 0.1g/kg, 0.2g/kg, na 0.3g/kg, n'otu n'otu.

1.3 Usoro Nnwale
1.3.1 Nnwale ihe ndọta: Họrọ azụ̀ carp ise (nke nwere nkezi ibu nke 30g) dị ka azụ̀ nnwale. Tupu ule ahụ, rie nri ruo awa 24, wee tinye azụ̀ nnwale ahụ n'ime akwarium iko (nke nwere nha 40 × 30 × 25cm). A na-edozi nri ndọta ahụ n'ebe dị anya nke 5.0cm site na ala akwarium ahụ site na iji ahịrị kwụ ọtọ nke e kegidere na ogwe kwụ ọtọ. Azụ̀ ahụ na-ata nri ahụ ma na-ama jijiji ahịrị ahụ, nke a na-ebuga na ogwe kwụ ọtọ ma dekọọ ya site na onye na-edekọ wiil. A na-agbakọ ugboro ole nri na-ata dabere na mkpọtụ kachasị elu nke azụ̀ ise na-ata nri ahụ n'ime nkeji 2. A na-eme ule nri maka otu nri ọ bụla ugboro atọ, na-eji bọọlụ ihe ndọta nri a kwadebere ọhụrụ oge ọ bụla. Site n'ime nnwale ugboro ugboro iji nweta ọnụọgụgụ na nkezi ugboro ole nri na-ata, mmetụta nri nkeDMTenwere ike inyocha DMPT na carp.

1.3.2 Nnwale uto ahụ na-eji akwarium iko asatọ (nha 55 × 45 × 50cm), yana omimi mmiri nke 40cm, okpomọkụ mmiri eke, na mmụba na-aga n'ihu. E kewara azụ̀ nnwale ahụ n'ụzọ na-enweghị usoro ma kewaa ha n'ime otu abụọ maka nnwale ahụ. Otu nke mbụ nwere akwarium anọ, X1 (otu njikwa), X2 (0.1gDMT/kg nri), X3 (0.2gDMT/kg nri), X4 (0.3gDMT/kg nri); Otu ọzọ nke akwarium anọ, nke nwere nọmba Y1 (otu njikwa), Y2 (0.10g nri DMPT/kg nri), Y3 (0.2g nri DMPT/kg nri), Y4 (0.30g nri DMPT/kg nri). Azụ 20 kwa igbe, a na-enye ha nri ugboro atọ kwa ụbọchị na elekere 8:00, elekere 13:00, na elekere 17:00, yana ọnụego nri kwa ụbọchị nke 5-7% nke ibu ahụ. Nnwale ahụ were izu isii. Ná mmalite na njedebe nke nnwale ahụ, a tụrụ oke mmiri nke azụ̀ nnwale ahụ ma dekọọ ọnụego ndụ nke otu ọ bụla.

2.1 Mmetụta nri nke DMPT naDMTn'elu azụ̀
Mmetụta nri nke DMPT naDMTA na-egosipụta ugboro ole azụ̀ nnwale ahụ na-ata n'oge nnwale nkeji abụọ, dịka egosiri na Tebụl 1. Nnwale ahụ chọpụtara na mgbe etinyere nri DMPT na DMT na akwarium, azụ̀ nnwale ahụ gosipụtara omume ịchọ nri ngwa ngwa, ebe mgbe ejiri nri otu njikwa mee ihe, mmeghachi omume nke azụ̀ nnwale ahụ dị nwayọ. Ma e jiri ya tụnyere nri njikwa, azụ̀ nnwale ahụ nwere mmụba dị ukwuu na ugboro ole ha na-ata nri nnwale ahụ. DMT na DMPT nwere mmetụta dị ukwuu na-adọrọ adọrọ na carp nnwale.

Ọnụọgụ ibu, ọnụego uto kpọmkwem, na ọnụego ndụ nke carp e nyere site na DMPT dị iche iche mụbara nke ukwuu ma e jiri ya tụnyere ndị e nyere nri njikwa, ebe ọnụọgụ nri belatara nke ukwuu. N'ime ha, mgbakwunye nke DMPT na T2, T3, na T4 mụbara mmụba ibu kwa ụbọchị nke otu atọ ahụ site na 52.94%, 78.43%, na 113.73%, n'otu n'otu, ma e jiri ya tụnyere otu njikwa. Ọnụọgụ ibu nke T2, T3, na T4 mụbara site na 60.44%, 73.85%, na 98.49%, n'otu n'otu, ọnụego uto kpọmkwem mụbara site na 41.22%, 51.15%, na 60.31%, n'otu n'otu. Ọnụọgụ ndụ mụbara site na 90% ruo 95%, ọnụọgụ nri belatara site na 28.01%, 29.41%, na 33.05%, n'otu n'otu.

3. Mmechi

N'ime nnwale a, ma ọ bụDMTma ọ bụ tinye DMPT, ugboro nri, ọnụego uto kpọmkwem, na mmụba ibu kwa ụbọchị nke azụ̀ nnwale n'ime otu ọ bụla mụbara nke ukwuu ma e jiri ya tụnyere otu njikwa, ebe ọnụọgụ nri belatara nke ukwuu. Ma ọ bụ DMT ma ọ bụ DMPT, mmetụta mmụba uto na-abawanye nke ukwuu ka mmụba nke ọnụọgụ mgbakwunye na mkpokọta atọ nke 0.1g/kg, 0.2g/kg, na 0.3g/kg. N'otu oge ahụ, e mere ntụnyere nke mmetụta nri na mmụba uto nke DMT na DMPT. A chọpụtara na n'okpuru otu mkpokọta ntutu isi, ugboro nri, ọnụego mmụba ibu, na ọnụego uto kpọmkwem nke azụ̀ nnwale na otu nri DMPT mụbara nke ukwuu ma e jiri ya tụnyere otu nri DMT, ebe ọnụọgụ nri belatara nke ukwuu. N'ikwu ya n'ụzọ dị iche, DMPT nwere mmetụta dị mkpa karị n'ịdọta na ịkwalite uto nke carp ma e jiri ya tụnyere DMT. Nnwale a jiri DMPT na DMT agbakwunyere na nri carp iji chọpụta mmetụta nri ha na ịkwalite uto. Nsonaazụ na-egosi na DMPT na DMT nwere atụmanya sara mbara maka ojiji dị ka ọgbọ ọhụrụ nke ndị na-adọta anụmanụ mmiri.